Is this blend one compound?
No. It contains two separately named components, BPC-157 and a TB-500-related substance. The calculator keeps their concentrations and identities separate.
BPC-157 + TB-500 Blend Calculator
Classification: A two-component research blend: BPC-157 and a TB-500-related thymosin beta-4 substance; not a single peptide.
Overview
This calculator treats BPC-157 and TB-500 as two distinct components in one labeled container. BPC-157 is described in the literature as a synthetic 15-amino-acid peptide. TB-500 is a label term used in FDA materials for a thymosin beta-4-related bulk substance; the shorthand should not automatically be treated as identical to full-length thymosin beta-4.
A blend has two concentrations because it has two component masses. The same liquid volume is used for both calculations, while each component has its own entered amount. The fixed component ratio is the BPC-157 mass relative to the TB-500 mass entered for that container.
Selecting BPC-157 as the reference means the entered BPC-157 mass determines the represented volume; the same volume necessarily represents a mathematically linked TB-500 mass. Selecting TB-500 reverses the reference. The two components cannot be selected independently from one shared volume.
Research context
BPC-157 publications include animal and review literature. Their experimental results do not establish that a BPC-157 and TB-500 combination has a clinical effect.
Thymosin beta-4 has appeared in registered clinical studies involving specific investigational products and conditions. A study of full-length thymosin beta-4 cannot be used automatically to validate a TB-500 label or this blend.
Evidence and regulatory status
The FDA meeting page identifies BPC-157-related and TB-500-related substances as separate items discussed for possible inclusion in a compounding list. Advisory discussion is not approval and does not make a blend an approved product.
Unit notes
Each component can be entered in mg or mcg, with 1 mg = 1,000 mcg used for normalization. The same liquid-volume entry is applied to both component concentration calculations.
The blend ratio is shown from the two normalized vial amounts. The reference-component amount divided by its concentration yields represented mL; the other component is that mL multiplied by its concentration.
U-100 means 100 units per mL and U-40 means 40 units per mL. These are display scales only. A syringe-unit result cannot identify either component’s mass without the corresponding concentration.
If the selected reference amount exceeds its entered component amount, the application reports the validation message rather than silently changing the ratio.
Limitations
- The calculator cannot verify the ratio printed on a label, distinguish salts or fragments, or determine whether both components are present. It cannot assess compatibility, purity, sterility, degradation, or product provenance.
- A fixed ratio means the output for the non-reference component follows the same represented volume. The interface does not provide a way to independently choose both component amounts for one shared volume.
Frequently asked questions
How does fixed-ratio blend arithmetic work?
One reference component determines the represented volume. That same volume mathematically represents the other component according to the two amounts entered for the container.
Can I independently select both component amounts?
No. A fixed blend provides one shared volume, so the two component amounts represented by that volume remain linked by the entered ratio.
Does the blend calculator establish that combining the components is useful?
No. It reports arithmetic only and does not establish effectiveness, compatibility, safety, or a clinical use for a combination.
Does PepLedger determine an amount for either component?
No. PepLedger does not determine or recommend an amount. It performs arithmetic using values entered by the visitor.
Why must BPC-157 and TB-500 labels be checked separately?
They are distinct substances with different names, evidence, and regulatory questions. Similar shorthand does not prove identical chemical identity.
What does U-100 mean for the blend?
U-100 is a display scale of 100 units per mL. It does not describe the blend ratio or either component’s mass.
Sources and official references
- July 23-24, 2026: Meeting of the Pharmacy Compounding Advisory Committee — U.S. Food and Drug Administration (2026)
- Hepatoprotective effect of BPC 157, a 15-amino acid peptide, on liver lesions — PubMed, U.S. National Library of Medicine (1993)
- Study of the Safety and Efficacy of Injectable Thymosin Beta 4 for Treating Acute Myocardial Infarction — ClinicalTrials.gov, U.S. National Library of Medicine
- Thymosin β4: a multi-functional regenerative peptide. Basic properties and clinical applications — PubMed, U.S. National Library of Medicine (2012)
Calculation methodology
PepLedger calculates concentration, liquid volume and syringe-scale position exclusively from the values you enter. It does not choose or recommend an amount.
View our calculation methodology and editorial standards
Last updated: September 13, 2026